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A Real-time Potency Assay for Chimeric Antigen Receptor T Cells Targeting Solid and Hematological Cancer Cells
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November 12, 2019
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We describe a quantitative real-time in vitro cytolysis assay system to evaluate the potency of chimeric antigen receptor T cells targeting liquid and solid tumor cells. This protocol can be extended to assess other immune effector cells, as well as combination treatments.
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Xi, B., Berahovich, R., Zhou, H., Xu, S., Wei, Y., Guan, J., Harto, H., Guan, J., Wu, L., Santa Ana, D., Cerignoil, F., Lamarche, B., Abassi, Y. A., Golubovskaya, V. A Real-time Potency Assay for Chimeric Antigen Receptor T Cells Targeting Solid and Hematological Cancer Cells. J. Vis. Exp. (153), e59033, doi:10.3791/59033 (2019).
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