Bioluminescence Resonance Energy Transfer (BRET)-Based Assay for Measuring Interactions of CRAF with 14-3-3 Proteins in Live Cells

Russell Spencer-Smith

doi:10.3791/66436

Journal

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Biochemistry

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Ensayo basado en transferencia de energía por resonancia de bioluminiscencia (BRET) para medir las interacciones de CRAF con proteínas 14-3-3 en células vivas

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Biochemistry

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JoVE Journal Biochemistry

Bioluminescence Resonance Energy Transfer (BRET)-Based Assay for Measuring Interactions of CRAF with 14-3-3 Proteins in Live Cells

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Ensayo basado en transferencia de energía por resonancia de bioluminiscencia (BRET) para medir las interacciones de CRAF con proteínas 14-3-3 en células vivas

396 Views

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01:07 min

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March 01, 2024

DOI:

10.3791/66436-v

DOI:

10.3791/66436-v

•

01:07 min

March 01, 2024

•

19 Views

Russell Spencer-Smith^1,2,3

¹Department of Cell and Molecular Pharmacology and Experimental Therapeutics,Medical University of South Carolina, ²Hollings Cancer Center,Medical University of South Carolina, ³Laboratory of Cell and Developmental Signaling, Center for Cancer Research,National Cancer Institute-Frederick

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Ensayo basado en transferencia de energía por resonancia de bioluminiscencia (BRET) para medir las interacciones de CRAF con proteínas 14-3-3 en células vivas

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