Journal
/
生物化学
/
CRAF-14-3-3 Interaction Analysis in Live Cells Using a BRET-Based Approach
/
Author Spotlight: Integrating BRET-Based Assays and Rare Mutation Analysis to Decipher RAF Kinase Regulation in Live Cells
JoVE 杂志
生物化学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 生物化学
Bioluminescence Resonance Energy Transfer (BRET)-Based Assay for Measuring Interactions of CRAF with 14-3-3 Proteins in Live Cells

Author Spotlight: Integrating BRET-Based Assays and Rare Mutation Analysis to Decipher RAF Kinase Regulation in Live Cells

383 Views

•

01:07 min

•

March 01, 2024

DOI:

10.3791/66436-v

DOI:
10.3791/66436-v

•

01:07 min
March 01, 2024

•

19 Views
1Department of Cell and Molecular Pharmacology and Experimental Therapeutics,Medical University of South Carolina, 2Hollings Cancer Center,Medical University of South Carolina, 3Laboratory of Cell and Developmental Signaling, Center for Cancer Research,National Cancer Institute-Frederick

Explore More Videos

生物化学CRAFRAF KinaseRAS GTPasesLive Cell AssayProtein-protein InteractionsKRAS-driven CancersNoonan SyndromeRAF DimerizationRAF AutoinhibitionRAF FunctionTherapeutic Targeting

Read Article

Cite this Article

Spencer-Smith, R. Bioluminescence Resonance Energy Transfer (BRET)-Based Assay for Measuring Interactions of CRAF with 14-3-3 Proteins in Live Cells. J. Vis. Exp. (205), e66436, doi:10.3791/66436 (2024).

Download .ris file

Copy

Share Video

.

Copy