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CRAF-14-3-3 Interaction Analysis in Live Cells Using a BRET-Based Approach
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Saggio basato sul trasferimento di energia per risonanza di bioluminescenza (BRET) per la misurazione delle interazioni di CRAF con proteine 14-3-3 in cellule vive
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Bioluminescence Resonance Energy Transfer (BRET)-Based Assay for Measuring Interactions of CRAF with 14-3-3 Proteins in Live Cells
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Saggio basato sul trasferimento di energia per risonanza di bioluminescenza (BRET) per la misurazione delle interazioni di CRAF con proteine 14-3-3 in cellule vive

italiano

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396 Views

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01:07 min

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March 01, 2024

DOI:

10.3791/66436-v

DOI:
10.3791/66436-v

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01:07 min
March 01, 2024

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19 Views
1Department of Cell and Molecular Pharmacology and Experimental Therapeutics,Medical University of South Carolina, 2Hollings Cancer Center,Medical University of South Carolina, 3Laboratory of Cell and Developmental Signaling, Center for Cancer Research,National Cancer Institute-Frederick

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Spencer-Smith, R. Bioluminescence Resonance Energy Transfer (BRET)-Based Assay for Measuring Interactions of CRAF with 14-3-3 Proteins in Live Cells. J. Vis. Exp. (205), e66436, doi:10.3791/66436 (2024).

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