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Author Spotlight: Integrating BRET-Based Assays and Rare Mutation Analysis to Decipher RAF Kinase Regulation in Live Cells

JoVE Revista
Bioquímica

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JoVE Revista Bioquímica

Bioluminescence Resonance Energy Transfer (BRET)-Based Assay for Measuring Interactions of CRAF with 14-3-3 Proteins in Live Cells

Author Spotlight: Integrating BRET-Based Assays and Rare Mutation Analysis to Decipher RAF Kinase Regulation in Live Cells

349 Views

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01:07 min

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March 01, 2024

DOI:

10.3791/66436-v

DOI:

10.3791/66436-v

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01:07 min

March 01, 2024

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6 Views

Russell Spencer-Smith^2,3

¹Department of Cell and Molecular Pharmacology and Experimental Therapeutics,Medical University of South Carolina, ²Hollings Cancer Center,Medical University of South Carolina, ³Laboratory of Cell and Developmental Signaling, Center for Cancer Research,National Cancer Institute-Frederick

Tags

Bioquímica CRAF RAF Kinase RAS GTPases Live Cell Assay Protein-protein Interactions KRAS-driven Cancers Noonan Syndrome RAF Dimerization RAF Autoinhibition RAF Function Therapeutic Targeting

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