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CRAF-14-3-3 Interaction Analysis in Live Cells Using a BRET-Based Approach
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Biolumineszenz-Resonanz-Energietransfer (BRET)-basierter Assay zur Messung der Wechselwirkungen von CRAF mit 14-3-3-Proteinen in lebenden Zellen
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Biochemistry
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JoVE Journal Biochemistry
Bioluminescence Resonance Energy Transfer (BRET)-Based Assay for Measuring Interactions of CRAF with 14-3-3 Proteins in Live Cells
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Biolumineszenz-Resonanz-Energietransfer (BRET)-basierter Assay zur Messung der Wechselwirkungen von CRAF mit 14-3-3-Proteinen in lebenden Zellen

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396 Views

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01:07 min

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March 01, 2024

DOI:

10.3791/66436-v

DOI:
10.3791/66436-v

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01:07 min
March 01, 2024

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19 Views
1Department of Cell and Molecular Pharmacology and Experimental Therapeutics,Medical University of South Carolina, 2Hollings Cancer Center,Medical University of South Carolina, 3Laboratory of Cell and Developmental Signaling, Center for Cancer Research,National Cancer Institute-Frederick

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Spencer-Smith, R. Bioluminescence Resonance Energy Transfer (BRET)-Based Assay for Measuring Interactions of CRAF with 14-3-3 Proteins in Live Cells. J. Vis. Exp. (205), e66436, doi:10.3791/66436 (2024).

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