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CRAF-14-3-3 Interaction Analysis in Live Cells Using a BRET-Based Approach
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Ensaio baseado em transferência de energia por ressonância de bioluminescência (BRET) para medir interações de CRAF com proteínas 14-3-3 em células vivas
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Bioluminescence Resonance Energy Transfer (BRET)-Based Assay for Measuring Interactions of CRAF with 14-3-3 Proteins in Live Cells

Ensaio baseado em transferência de energia por ressonância de bioluminescência (BRET) para medir interações de CRAF com proteínas 14-3-3 em células vivas

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396 Views

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01:07 min

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March 01, 2024

DOI:

10.3791/66436-v

DOI:
10.3791/66436-v

•

01:07 min
March 01, 2024

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19 Views
1Department of Cell and Molecular Pharmacology and Experimental Therapeutics,Medical University of South Carolina, 2Hollings Cancer Center,Medical University of South Carolina, 3Laboratory of Cell and Developmental Signaling, Center for Cancer Research,National Cancer Institute-Frederick

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Spencer-Smith, R. Bioluminescence Resonance Energy Transfer (BRET)-Based Assay for Measuring Interactions of CRAF with 14-3-3 Proteins in Live Cells. J. Vis. Exp. (205), e66436, doi:10.3791/66436 (2024).

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