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Journal
/
Biochemistry
/
CRAF-14-3-3 Interaction Analysis in Live Cells Using a BRET-Based Approach
/
Author Spotlight: Integrating BRET-Based Assays and Rare Mutation Analysis to Decipher RAF Kinase Regulation in Live Cells
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Bioluminescence Resonance Energy Transfer (BRET)-Based Assay for Measuring Interactions of CRAF with 14-3-3 Proteins in Live Cells
Author Spotlight: Integrating BRET-Based Assays and Rare Mutation Analysis to Decipher RAF Kinase Regulation in Live Cells
CRAF-14-3-3 Interaction Analysis in Live Cells Using a BRET-Based Approach
DOI:
10.3791/66436-v
•
01:07 min
•
March 01, 2024
•
Russell Spencer-Smith
2,3
1
Department of Cell and Molecular Pharmacology and Experimental Therapeutics
,
Medical University of South Carolina
,
2
Hollings Cancer Center
,
Medical University of South Carolina
,
3
Laboratory of Cell and Developmental Signaling, Center for Cancer Research
,
National Cancer Institute-Frederick
Tags
Biochemistry
CRAF
RAF Kinase
RAS GTPases
Live Cell Assay
Protein-protein Interactions
KRAS-driven Cancers
Noonan Syndrome
RAF Dimerization
RAF Autoinhibition
RAF Function
Therapeutic Targeting
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