Journal
/
Biochemistry
/
CRAF-14-3-3 Interaction Analysis in Live Cells Using a BRET-Based Approach
/
Dosage basé sur le transfert d’énergie par résonance de bioluminescence (BRET) pour mesurer les interactions de CRAF avec les protéines 14-3-3 dans les cellules vivantes
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
Bioluminescence Resonance Energy Transfer (BRET)-Based Assay for Measuring Interactions of CRAF with 14-3-3 Proteins in Live Cells
Please note that all translations are automatically generated. Click here for the English version.

Dosage basé sur le transfert d’énergie par résonance de bioluminescence (BRET) pour mesurer les interactions de CRAF avec les protéines 14-3-3 dans les cellules vivantes

français

Automatically Generated

396 Views

•

01:07 min

•

March 01, 2024

DOI:

10.3791/66436-v

DOI:
10.3791/66436-v

•

01:07 min
March 01, 2024

•

19 Views
1Department of Cell and Molecular Pharmacology and Experimental Therapeutics,Medical University of South Carolina, 2Hollings Cancer Center,Medical University of South Carolina, 3Laboratory of Cell and Developmental Signaling, Center for Cancer Research,National Cancer Institute-Frederick

Explore More Videos

BiochemistryCRAFRAF KinaseRAS GTPasesLive Cell AssayProtein-protein InteractionsKRAS-driven CancersNoonan SyndromeRAF DimerizationRAF AutoinhibitionRAF FunctionTherapeutic Targeting

Read Article

Cite this Article

Spencer-Smith, R. Bioluminescence Resonance Energy Transfer (BRET)-Based Assay for Measuring Interactions of CRAF with 14-3-3 Proteins in Live Cells. J. Vis. Exp. (205), e66436, doi:10.3791/66436 (2024).

Download .ris file

Copy

Share Video

.

Copy