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CRAF-14-3-3 Interaction Analysis in Live Cells Using a BRET-Based Approach
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Bioluminescence Resonance Energy Transfer (BRET) - בדיקה מבוססת למדידת אינטראקציות של CRAF עם 14-3-3 חלבונים בתאים חיים
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Biochemistry
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JoVE Journal Biochemistry
Bioluminescence Resonance Energy Transfer (BRET)-Based Assay for Measuring Interactions of CRAF with 14-3-3 Proteins in Live Cells
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Bioluminescence Resonance Energy Transfer (BRET) - בדיקה מבוססת למדידת אינטראקציות של CRAF עם 14-3-3 חלבונים בתאים חיים

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396 Views

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01:07 min

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March 01, 2024

DOI:

10.3791/66436-v

DOI:
10.3791/66436-v

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01:07 min
March 01, 2024

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19 Views
1Department of Cell and Molecular Pharmacology and Experimental Therapeutics,Medical University of South Carolina, 2Hollings Cancer Center,Medical University of South Carolina, 3Laboratory of Cell and Developmental Signaling, Center for Cancer Research,National Cancer Institute-Frederick

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Cite this Article

Spencer-Smith, R. Bioluminescence Resonance Energy Transfer (BRET)-Based Assay for Measuring Interactions of CRAF with 14-3-3 Proteins in Live Cells. J. Vis. Exp. (205), e66436, doi:10.3791/66436 (2024).

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