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Journal
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Immunology and Infection
/
从小鼠中分离和培养骨髓衍生树突状细胞的经济有效的方案
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Immunology and Infection
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Immunology and Infection
Economical and Efficient Protocol for Isolating and Culturing Bone Marrow-derived Dendritic Cells from Mice
Please note that all translations are automatically generated.
Click here for the English version.
从小鼠中分离和培养骨髓衍生树突状细胞的经济有效的方案
DOI:
10.3791/63125-v
•
04:29 min
•
July 01, 2022
•
Huiqin Tang*
1
,
Hui Xie*
1
,
Zhun Wang
,
Shuanghe Peng
,
Wenli Ni
,
Linpei Guo
1
Tianjin Institute of Urology
,
The Second Hospital of Tianjin Medical University
,
2
Department of Pathology
,
The Second Hospital of Tianjin Medical University
,
3
Department of Urology
,
The Affiliated Wuxi No.2 People’s Hospital of Nanjing Medical University
Chapters
00:17
Introduction
00:37
Isolation of Bone Marrow and Preparation of BM Cells
02:05
Induction Culture of BMDC
03:11
Results
03:59
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们提出了一种经济有效的方法,在用10 ng / mL GM-CSF / IL-4培养7天后从小鼠中分离和产生高纯度骨髓来源的树突状细胞。
Tags
Bone Marrow-derived Dendritic Cells
Isolation
Culture
Mouse
BMDC
CD11c
CD80
MSC2
GM-CSF
IL-4
Cost-effective
Efficient
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