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マウスから骨髄由来樹状細胞を単離および培養するための経済的かつ効率的なプロトコル
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Immunology and Infection
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JoVE Journal Immunology and Infection
Economical and Efficient Protocol for Isolating and Culturing Bone Marrow-derived Dendritic Cells from Mice
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マウスから骨髄由来樹状細胞を単離および培養するための経済的かつ効率的なプロトコル

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6,957 Views

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04:29 min

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July 01, 2022

DOI:

10.3791/63125-v

DOI:
10.3791/63125-v

•

04:29 min
July 01, 2022

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38 Views
, , , , ,
1Tianjin Institute of Urology,The Second Hospital of Tianjin Medical University, 2Department of Pathology,The Second Hospital of Tianjin Medical University, 3Department of Urology,The Affiliated Wuxi No.2 People’s Hospital of Nanjing Medical University

Summary

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ここでは、10 ng/mL GM-CSF/IL-4で7日間培養した後、マウスから高純度骨髄由来樹状細胞を単離・生成する経済的かつ効率的な方法を紹介します。

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Tang, H., Xie, H., Wang, Z., Peng, S., Ni, W., Guo, L. Economical and Efficient Protocol for Isolating and Culturing Bone Marrow-derived Dendritic Cells from Mice. J. Vis. Exp. (185), e63125, doi:10.3791/63125 (2022).

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