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Protocollo economico ed efficiente per l'isolamento e la coltura di cellule dendritiche derivate dal midollo osseo da topi
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Immunology and Infection
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JoVE Journal Immunology and Infection
Economical and Efficient Protocol for Isolating and Culturing Bone Marrow-derived Dendritic Cells from Mice
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Protocollo economico ed efficiente per l'isolamento e la coltura di cellule dendritiche derivate dal midollo osseo da topi

italiano

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7,092 Views

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04:29 min

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July 01, 2022

DOI:

10.3791/63125-v

DOI:
10.3791/63125-v

•

04:29 min
July 01, 2022

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38 Views
, , , , ,
1Tianjin Institute of Urology,The Second Hospital of Tianjin Medical University, 2Department of Pathology,The Second Hospital of Tianjin Medical University, 3Department of Urology,The Affiliated Wuxi No.2 People’s Hospital of Nanjing Medical University

Summary

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Qui, presentiamo un metodo economico ed efficiente per isolare e generare cellule dendritiche derivate dal midollo osseo ad alta purezza dai topi dopo 7 giorni di coltura con 10 ng / mL GM-CSF / IL-4.

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Cite this Article

Tang, H., Xie, H., Wang, Z., Peng, S., Ni, W., Guo, L. Economical and Efficient Protocol for Isolating and Culturing Bone Marrow-derived Dendritic Cells from Mice. J. Vis. Exp. (185), e63125, doi:10.3791/63125 (2022).

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