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Toxoplasma gondii Cyst Wall Formation in Activated Bone Marrow-derived Macrophages and Bradyzoite Conditions
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Chapters
- 00:04Title
- 00:44Introduction
- 01:10Preparation of HFF-coated Coverslips
- 02:37Infecting Cells
- 03:57Detection of Bradyzoites
- 05:48Inducing Cyst Wall Formation
- 09:08Representative Results
- 09:30Conclusion
Toxoplasma gondii converts to a cyst form in response to environmental stresses, which can be mimicked in tissue culture models. This video demonstrates techniques to examine cyst wall formation by activating bone marrow-derived macrophages or changing growth medium pH in fibroblast cells.
Tags
Toxoplasma GondiiCyst Wall FormationBone Marrow-derived MacrophagesBradyzoite ConditionsObligate Intracellular ParasiteTachyzoiteEncysted FormSignaling ProcessChronic Stage Of InfectionCO2 StarvationHigh PH MediumInterferon Gamma (IFNγ)Dolichos Biflorus Agglutinin (DBA)Fluorescently Labeled DBAHuman Foreskin Fibroblasts (HFFs)Murine Bone Marrow-derived Macrophages (BMMs)Lipopolysaccharide (LPS)High PH Growth Medium
