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بروتوكول اقتصادي وفعال لعزل وزراعة الخلايا الشجيرية المشتقة من نخاع العظم من الفئران
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Immunology and Infection
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JoVE Journal Immunology and Infection
Economical and Efficient Protocol for Isolating and Culturing Bone Marrow-derived Dendritic Cells from Mice
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بروتوكول اقتصادي وفعال لعزل وزراعة الخلايا الشجيرية المشتقة من نخاع العظم من الفئران

العربية

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7,092 Views

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04:29 min

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July 01, 2022

DOI:

10.3791/63125-v

DOI:
10.3791/63125-v

•

04:29 min
July 01, 2022

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38 Views
, , , , ,
1Tianjin Institute of Urology,The Second Hospital of Tianjin Medical University, 2Department of Pathology,The Second Hospital of Tianjin Medical University, 3Department of Urology,The Affiliated Wuxi No.2 People’s Hospital of Nanjing Medical University

Summary

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هنا ، نقدم طريقة اقتصادية وفعالة لعزل وتوليد خلايا تغصنية عالية النقاء مشتقة من نخاع العظام من الفئران بعد 7 أيام من الزراعة مع 10 نانوغرام / مل GM-CSF / IL-4.

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Cite this Article

Tang, H., Xie, H., Wang, Z., Peng, S., Ni, W., Guo, L. Economical and Efficient Protocol for Isolating and Culturing Bone Marrow-derived Dendritic Cells from Mice. J. Vis. Exp. (185), e63125, doi:10.3791/63125 (2022).

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