Journal
/
免疫与感染
/
Economical and Efficient Protocol for Isolating and Culturing Bone Marrow-derived Dendritic Cells from Mice
JoVE 杂志
免疫与感染
Author Produced
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 免疫与感染
Economical and Efficient Protocol for Isolating and Culturing Bone Marrow-derived Dendritic Cells from Mice

Economical and Efficient Protocol for Isolating and Culturing Bone Marrow-derived Dendritic Cells from Mice

7,101 Views

•

04:29 min

•

July 01, 2022

DOI:

10.3791/63125-v

DOI:
10.3791/63125-v

•

04:29 min
July 01, 2022

•

38 Views
, , , , ,
1Tianjin Institute of Urology,The Second Hospital of Tianjin Medical University, 2Department of Pathology,The Second Hospital of Tianjin Medical University, 3Department of Urology,The Affiliated Wuxi No.2 People’s Hospital of Nanjing Medical University

Summary

Automatically generated

Here, we present an economical and efficient method to isolate and generate high-purity bone marrow-derived dendritic cells from mice after 7 days of culture with 10 ng/mL GM-CSF/IL-4.

Explore More Videos

Bone Marrow-derived Dendritic CellsIsolationCultureMouseBMDCCD11cCD80MSC2GM-CSFIL-4Cost-effectiveEfficient

Read Article

Cite this Article

Tang, H., Xie, H., Wang, Z., Peng, S., Ni, W., Guo, L. Economical and Efficient Protocol for Isolating and Culturing Bone Marrow-derived Dendritic Cells from Mice. J. Vis. Exp. (185), e63125, doi:10.3791/63125 (2022).

Download .ris file

Copy

Share Video

.

Copy