Journal
/
Immunology and Infection
/
Fluorescence-activated Cell Sorting for Purification of Plasmacytoid Dendritic Cells from the Mouse Bone Marrow
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Fluorescence-activated Cell Sorting for Purification of Plasmacytoid Dendritic Cells from the Mouse Bone Marrow

Fluorescence-activated Cell Sorting for Purification of Plasmacytoid Dendritic Cells from the Mouse Bone Marrow

English

Automatically Generated

11,820 Views

•

08:01 min

•

November 04, 2016

DOI:

10.3791/54641-v

DOI:
10.3791/54641-v

•

08:01 min
November 04, 2016

•

13 Views
, ,
1Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine,Virginia Polytechnic Institute and State University, 2Flow Cytometry Laboratory, Virginia-Maryland College of Veterinary Medicine,Virginia Polytechnic Institute and State University

Summary

Automatically generated

We report a protocol using fluorescence-activated cell sorting to isolate plasmacytoid dendritic cells (pDC) with high purity from the bone marrow of lupus-prone mice for functional studies of pDC.

Explore More Videos

Fluorescence-activated Cell SortingPlasmacytoid Dendritic CellsBone MarrowLupusImmune CellInterferon AlphaDensity GradientCell Surface StainingFACS

Read Article

Cite this Article

Liao, X., Makris, M., Luo, X. M. Fluorescence-activated Cell Sorting for Purification of Plasmacytoid Dendritic Cells from the Mouse Bone Marrow. J. Vis. Exp. (117), e54641, doi:10.3791/54641 (2016).

Download .ris file

Copy

Share Video

.

Copy