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Economisch en efficiënt protocol voor het isoleren en kweken van beenmerg-afgeleide dendritische cellen van muizen
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Immunology and Infection
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JoVE Journal Immunology and Infection
Economical and Efficient Protocol for Isolating and Culturing Bone Marrow-derived Dendritic Cells from Mice
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Economisch en efficiënt protocol voor het isoleren en kweken van beenmerg-afgeleide dendritische cellen van muizen

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7,092 Views

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04:29 min

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July 01, 2022

DOI:

10.3791/63125-v

DOI:
10.3791/63125-v

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04:29 min
July 01, 2022

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38 Views
, , , , ,
1Tianjin Institute of Urology,The Second Hospital of Tianjin Medical University, 2Department of Pathology,The Second Hospital of Tianjin Medical University, 3Department of Urology,The Affiliated Wuxi No.2 People’s Hospital of Nanjing Medical University

Summary

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Hier presenteren we een economische en efficiënte methode om zeer zuivere beenmerg-afgeleide dendritische cellen van muizen te isoleren en te genereren na 7 dagen cultuur met 10 ng / ml GM-CSF / IL-4.

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Tang, H., Xie, H., Wang, Z., Peng, S., Ni, W., Guo, L. Economical and Efficient Protocol for Isolating and Culturing Bone Marrow-derived Dendritic Cells from Mice. J. Vis. Exp. (185), e63125, doi:10.3791/63125 (2022).

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