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Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages

Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages

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10:07 min

June 23, 2013

DOI:

10:07 min
June 23, 2013

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Summary

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The article describes a readily easy adaptive in vitro model to investigate macrophage polarization. In the presence of GM-CSF/M-CSF, hematopoietic stem/progenitor cells from the bone marrow are directed into monocytic differentiation, followed by M1 or M2 stimulation. The activation status can be tracked by changes in cell surface antigens, gene expression and cell signaling pathways.

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