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Validando o sequenciamento de nanoporos de genoma inteiro, usando o vírus Usutu como exemplo
JoVE Journal
Genetics
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JoVE Journal Genetics
Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example

Validando o sequenciamento de nanoporos de genoma inteiro, usando o vírus Usutu como exemplo

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8,292 Views

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05:45 min

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March 11, 2020

DOI:

10.3791/60906-v

DOI:
10.3791/60906-v

•

05:45 min
March 11, 2020

•

13 Views
, , ,
1ErasmusMC, Department of Viroscience, WHO Collaborating Centre for Arbovirus and Viral Hemorrhagic Fever Reference and Research,Erasmus University Medical Center

Summary

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Nós validamos anteriormente um protocolo para sequenciamento do vírus Usutu (USUV) baseado em amplicon em uma plataforma de sequenciamento de nanoporos. Aqui, descrevemos os métodos usados com mais detalhes e determinamos a taxa de erro da célula de fluxo Nanopore R10.

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Oude Munnink, B. B., Nieuwenhuijse, D. F., Sikkema, R. S., Koopmans, M. Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example. J. Vis. Exp. (157), e60906, doi:10.3791/60906 (2020).

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