Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example

Bas  B. Oude Munnink; David  F. Nieuwenhuijse; Reina  S. Sikkema; Marion Koopmans

doi:10.3791/60906

Journal

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Genetics

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Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example

JoVE Journal
Genetics

This content is Free Access.

JoVE Journal Genetics

Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example

8,289 Views

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05:45 min

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March 11, 2020

DOI:

10.3791/60906-v

DOI:

10.3791/60906-v

•

05:45 min

March 11, 2020

•

13 Views

Bas B. Oude Munnink¹, David F. Nieuwenhuijse¹, Reina S. Sikkema¹, Marion Koopmans¹

¹ErasmusMC, Department of Viroscience, WHO Collaborating Centre for Arbovirus and Viral Hemorrhagic Fever Reference and Research,Erasmus University Medical Center

Summary

We previously validated a protocol for amplicon-based whole genome Usutu virus (USUV) sequencing on a nanopore sequencing platform. Here, we describe the methods used in more detail and determine the error rate of the nanopore R10 flow cell.

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Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example

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