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Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example
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Genética
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JoVE Revista Genética
Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example

Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example

8,289 Views

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05:45 min

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March 11, 2020

DOI:

10.3791/60906-v

DOI:
10.3791/60906-v

•

05:45 min
March 11, 2020

•

13 Views
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1ErasmusMC, Department of Viroscience, WHO Collaborating Centre for Arbovirus and Viral Hemorrhagic Fever Reference and Research,Erasmus University Medical Center

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We previously validated a protocol for amplicon-based whole genome Usutu virus (USUV) sequencing on a nanopore sequencing platform. Here, we describe the methods used in more detail and determine the error rate of the nanopore R10 flow cell.

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Oude Munnink, B. B., Nieuwenhuijse, D. F., Sikkema, R. S., Koopmans, M. Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example. J. Vis. Exp. (157), e60906, doi:10.3791/60906 (2020).

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