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Validering van Whole Genome Nanopore Sequencing, met behulp van Usutu Virus als voorbeeld
JoVE Journal
Genetics
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JoVE Journal Genetics
Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example
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Validering van Whole Genome Nanopore Sequencing, met behulp van Usutu Virus als voorbeeld

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8,292 Views

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05:45 min

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March 11, 2020

DOI:

10.3791/60906-v

DOI:
10.3791/60906-v

•

05:45 min
March 11, 2020

•

13 Views
, , ,
1ErasmusMC, Department of Viroscience, WHO Collaborating Centre for Arbovirus and Viral Hemorrhagic Fever Reference and Research,Erasmus University Medical Center

Summary

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We valideerden eerder een protocol voor op amplicon gebaseerde whole genome Usutu virus (USUV) sequencing op een nanopore sequencing platform. Hier beschrijven we de methoden die in meer detail worden gebruikt en bepalen we het foutenpercentage van de nanopore R10-stroomcel.

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Oude Munnink, B. B., Nieuwenhuijse, D. F., Sikkema, R. S., Koopmans, M. Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example. J. Vis. Exp. (157), e60906, doi:10.3791/60906 (2020).

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