Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example

Bas  B. Oude Munnink; David  F. Nieuwenhuijse; Reina  S. Sikkema; Marion Koopmans

doi:10.3791/60906

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遺伝学

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Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example

JoVE Journal
遺伝学

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JoVE Journal 遺伝学

Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example

8,249 Views

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05:45 min

•

March 11, 2020

DOI:

10.3791/60906-v

DOI:

10.3791/60906-v

•

05:45 min

March 11, 2020

•

13 Views

Bas B. Oude Munnink¹, David F. Nieuwenhuijse¹, Reina S. Sikkema¹, Marion Koopmans¹

¹ErasmusMC, Department of Viroscience, WHO Collaborating Centre for Arbovirus and Viral Hemorrhagic Fever Reference and Research,Erasmus University Medical Center

概要

We previously validated a protocol for amplicon-based whole genome Usutu virus (USUV) sequencing on a nanopore sequencing platform. Here, we describe the methods used in more detail and determine the error rate of the nanopore R10 flow cell.

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Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example

概要

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