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全ゲノムナノポアシーケンシングの検証、例としてUsutuウイルスを用いた
JoVE Journal
Genetics
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JoVE Journal Genetics
Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example
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全ゲノムナノポアシーケンシングの検証、例としてUsutuウイルスを用いた

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8,285 Views

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05:45 min

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March 11, 2020

DOI:

10.3791/60906-v

DOI:
10.3791/60906-v

•

05:45 min
March 11, 2020

•

13 Views
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1ErasmusMC, Department of Viroscience, WHO Collaborating Centre for Arbovirus and Viral Hemorrhagic Fever Reference and Research,Erasmus University Medical Center

Summary

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我々は、ナノポアシーケンシングプラットフォーム上でアンプリコンベースの全ゲノムウストゥウイルス(USUV)シーケンシングのプロトコルを検証した。ここでは、ナノポアR10フローセルの誤差率をより詳細に用いた方法について説明する。

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Cite this Article

Oude Munnink, B. B., Nieuwenhuijse, D. F., Sikkema, R. S., Koopmans, M. Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example. J. Vis. Exp. (157), e60906, doi:10.3791/60906 (2020).

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