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Journal
/
Biochemistry
/
化学感受器配体结合域的高效复用与纯化方法
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain
Please note that all translations are automatically generated.
Click here for the English version.
化学感受器配体结合域的高效复用与纯化方法
DOI:
10.3791/57092-v
•
14:25 min
•
December 12, 2017
•
Mayra A. Machuca
2
,
Anna Roujeinikova
2,3
1
Infection and Immunity Program
,
Monash Biomedicine Discovery Institute
,
2
Department of Microbiology
,
Monash University
,
3
Department of Biochemistry and Molecular Biology
,
Monash University
Chapters
00:05
Title
00:46
Expression of His
6
-TIp3-LBD in
E. coli
02:29
Isolation and Denaturation of Inclusion Bodies
05:34
Protein Refolding
07:39
Purification of His
6
-tagged Protein Using Immobilized Metal Ion Affinity Chromatogrphy
08:49
His
6
-tag Removal Using TEV Protease and Size-excluson Chromatography of Tlp3-LBD
11:06
Results: Expression, Refolding, and Purification of Tlp3-LBD
12:41
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
提出了一种 dCACHE 周质配体结合域的复用过程, 该方法由包涵体化学感受器 Tlp3 和纯化, 产生毫克量的蛋白质.
Tags
Chemoreceptor
Ligand Binding Domain
Refolding
Purification
Inclusion Bodies
E. Coli
Protein Expression
IPTG Induction
Cell Lysis
Homogenization
Centrifugation
SDS-PAGE
Buffer Exchange
Solubilization
Protein Purification
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