/

/

高効率巻き戻しと受容体リガンド結合領域の浄化法

JoVE Journal
Biochemistry

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Biochemistry

Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain

Please note that all translations are automatically generated. Click here for the English version.

高効率巻き戻しと受容体リガンド結合領域の浄化法

17,420 Views

•

14:25 min

•

December 12, 2017

DOI:

10.3791/57092-v

DOI:

10.3791/57092-v

•

14:25 min

December 12, 2017

•

15 Views

Mayra A. Machuca^1,2, Anna Roujeinikova^1,2,3

¹Infection and Immunity Program,Monash Biomedicine Discovery Institute, ²Department of Microbiology,Monash University, ³Department of Biochemistry and Molecular Biology,Monash University

Summary

封入体、ミリグラム単位の微量のタンパク質を生成するための精製からカンピロバクター走 Tlp3 の dCACHE ペリプラズムリガンド結合ドメインの巻き戻しプロシージャが表示されます。

Explore More Videos

Chemoreceptor Ligand Binding Domain Refolding Purification Inclusion Bodies E. Coli Protein Expression IPTG Induction Cell Lysis Homogenization Centrifugation SDS-PAGE Buffer Exchange Solubilization Protein Purification

Read Article