Journal
/
Biochemistry
/
Methode für effiziente Umfaltung und Reinigung des Chemorezeptor-Liganden-bindende Domäne
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain
Please note that all translations are automatically generated. Click here for the English version.

Methode für effiziente Umfaltung und Reinigung des Chemorezeptor-Liganden-bindende Domäne

Deutsch

Automatically Generated

17,420 Views

•

14:25 min

•

December 12, 2017

DOI:

10.3791/57092-v

DOI:
10.3791/57092-v

•

14:25 min
December 12, 2017

•

15 Views
,
1Infection and Immunity Program,Monash Biomedicine Discovery Institute, 2Department of Microbiology,Monash University, 3Department of Biochemistry and Molecular Biology,Monash University

Summary

Automatically generated

Eine Prozedur wird vorgestellt, für die Umfaltung der dCACHE periplasmatischen Liganden-bindende Domäne von Campylobacter Jejuni Chemorezeptor Tlp3 Einschlusskörperchen und die Reinigung zu Milligramm-Mengen des Proteins führen.

Explore More Videos

ChemoreceptorLigand Binding DomainRefoldingPurificationInclusion BodiesE. ColiProtein ExpressionIPTG InductionCell LysisHomogenizationCentrifugationSDS-PAGEBuffer ExchangeSolubilizationProtein Purification

Read Article

Cite this Article

Machuca, M. A., Roujeinikova, A. Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain. J. Vis. Exp. (130), e57092, doi:10.3791/57092 (2017).

Download .ris file

Copy

Share Video

.

Copy