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Procédé de repliement efficace et la Purification des chémorécepteurs Ligand Binding Domain
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Biochemistry
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JoVE Journal Biochemistry
Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain
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Procédé de repliement efficace et la Purification des chémorécepteurs Ligand Binding Domain

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17,420 Views

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14:25 min

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December 12, 2017

DOI:

10.3791/57092-v

DOI:
10.3791/57092-v

•

14:25 min
December 12, 2017

•

15 Views
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1Infection and Immunity Program,Monash Biomedicine Discovery Institute, 2Department of Microbiology,Monash University, 3Department of Biochemistry and Molecular Biology,Monash University

Summary

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Une procédure est présentée pour le repliement de la domaine de liaison du ligand périplasmique dCACHE de Campylobacter jejuni zone chémoréceptrice Tlp3 de corps d’inclusion et de la purification pour produire des quantités de milligramme de protéine.

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Cite this Article

Machuca, M. A., Roujeinikova, A. Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain. J. Vis. Exp. (130), e57092, doi:10.3791/57092 (2017).

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