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Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions
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Biochemistry
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JoVE Journal Biochemistry
Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

English

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7,007 Views

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06:01 min

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January 07, 2019

DOI:

10.3791/58451-v

DOI:
10.3791/58451-v

•

06:01 min
January 07, 2019

•

5 Views
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1Receptor Discovery group, Microchemistry, Proteomics and Lipidomics Department,Genentech, 2Portfolio Management and Operations,Genentech

Summary

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Here, we present a protocol to screen extracellular protein microarrays for identification of novel receptor-ligand interactions in high throughput. We also describe a method to enhance detection of transient protein-protein interactions by using protein-microbead complexes.

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Extracellular Protein MicroarrayHigh Throughput DetectionLow Affinity Receptor-ligand InteractionsProtein-protein InteractionsMicro ArrayerEpoxy Silane SlidesCy3 Labeled BSABlocking SolutionMultivalent ApproachFc-tagged Extracellular DomainsProtein A Coated MicrobeadsCy5 IgG Labeling

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Husain, B., Paduchuri, S., Ramani, S. R., Martinez-Martin, N. Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions. J. Vis. Exp. (143), e58451, doi:10.3791/58451 (2019).

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