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Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain
JoVE Journal
Biochemie
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JoVE Journal Biochemie
Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain

Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain

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14:25 min

December 12, 2017

DOI:

14:25 min
December 12, 2017

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Summary

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A procedure is presented for the refolding of the dCACHE periplasmic ligand binding domain of Campylobacter jejuni chemoreceptor Tlp3 from inclusion bodies and the purification to yield milligram quantities of protein.

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