Journal
/
Biochemistry
/
שיטת Refolding יעילה וטיהור של ליגנד Chemoreceptor מחייב תחום
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain
Please note that all translations are automatically generated. Click here for the English version.

שיטת Refolding יעילה וטיהור של ליגנד Chemoreceptor מחייב תחום

עברית

Automatically Generated

17,420 Views

•

14:25 min

•

December 12, 2017

DOI:

10.3791/57092-v

DOI:
10.3791/57092-v

•

14:25 min
December 12, 2017

•

15 Views
,
1Infection and Immunity Program,Monash Biomedicine Discovery Institute, 2Department of Microbiology,Monash University, 3Department of Biochemistry and Molecular Biology,Monash University

Summary

Automatically generated

הליך זה הציג את refolding של התחום מחייב ליגנד periplasmic dCACHE של קמפילובקטר jejuni chemoreceptor Tlp3 מן ההכללה וגופי הטיהור להניב כמויות מיליגרם של חלבון.

Explore More Videos

ChemoreceptorLigand Binding DomainRefoldingPurificationInclusion BodiesE. ColiProtein ExpressionIPTG InductionCell LysisHomogenizationCentrifugationSDS-PAGEBuffer ExchangeSolubilizationProtein Purification

Read Article

Cite this Article

Machuca, M. A., Roujeinikova, A. Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain. J. Vis. Exp. (130), e57092, doi:10.3791/57092 (2017).

Download .ris file

Copy

Share Video

.

Copy