Journal
/
生物化学
/
Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain
JoVE 杂志
生物化学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 生物化学
Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain

Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain

17,373 Views

•

14:25 min

•

December 12, 2017

DOI:

10.3791/57092-v

DOI:
10.3791/57092-v

•

14:25 min
December 12, 2017

•

5 Views
,
1Infection and Immunity Program,Monash Biomedicine Discovery Institute, 2Department of Microbiology,Monash University, 3Department of Biochemistry and Molecular Biology,Monash University

Explore More Videos

ChemoreceptorLigand Binding DomainRefoldingPurificationInclusion BodiesE. ColiProtein ExpressionIPTG InductionCell LysisHomogenizationCentrifugationSDS-PAGEBuffer ExchangeSolubilizationProtein Purification

Read Article

Cite this Article

Machuca, M. A., Roujeinikova, A. Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain. J. Vis. Exp. (130), e57092, doi:10.3791/57092 (2017).

Download .ris file

Copy

Share Video

.

Copy