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Identification of Plant Ice-binding Proteins Through Assessment of Ice-recrystallization Inhibition and Isolation Using Ice-affinity Purification
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Biochemistry
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JoVE Journal Biochemistry
Identification of Plant Ice-binding Proteins Through Assessment of Ice-recrystallization Inhibition and Isolation Using Ice-affinity Purification

Identification of Plant Ice-binding Proteins Through Assessment of Ice-recrystallization Inhibition and Isolation Using Ice-affinity Purification

DOI:
10.3791/55302-v

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09:43 min

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May 05, 2017

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, ,
1Department of Biology,Queen’s University, 2Department of Biomedical and Molecular Sciences,Queen’s University, 3School of Environmental Sciences,Queen’s University

Chapters

  • 00:05Title
  • 00:45Preparation of Native Protein Extracts for Ice-recrystalization Inhibition (IRI) Analysis
  • 02:30Splat Assay Pre-experiment Set Up and Conducting the Splat Assay
  • 05:32Preparation of Samples for Ice-affinity Purification
  • 06:36Conducting Ice-affinity Purification
  • 08:14Results: Ice-hemispheres Grown Using IBP Extracts Collected from the Freeze-tolerant Grass, L. perenne
  • 09:15Conclusion

Summary

Automatic Translation

Automatic Translation

This paper outlines the identification of ice-binding proteins from freeze-tolerant plants through the assessment of ice-recrystallization inhibition activity and subsequent isolation of native IBPs using ice-affinity purification.

Tags

Keywords: Ice-binding ProteinsIBPsPlant Freeze ToleranceIce-affinity PurificationIce-recrystallization InhibitionCold-acclimationPlant ExtractProtein ExtractionCentrifugationIce-binding Activity

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