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Environment
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斑马鱼细胞系的细胞毒性测定
JoVE Journal
Environment
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Environment
Cytotoxicity Assays with Zebrafish Cell Lines
Please note that all translations are automatically generated.
Click here for the English version.
斑马鱼细胞系的细胞毒性测定
DOI:
10.3791/64860-v
•
08:22 min
•
January 06, 2023
•
Irisdoris Rodrigues de Souza
,
Tainá Wilke Sivek
,
Júlia Beatriz Vaz de Oliveira
,
Andrezza Di Pietro Micali Canavez
,
Natália de Albuquerque Vita
,
Desiree Cigaran Schuck
,
Isisdoris Rodrigues de Souza
,
Marta Margarete Cestari
,
Marcio Lorencini
,
Daniela Morais Leme
1
Graduate Program in Genetics, Department of Genetics
,
Federal University of Paraná (UFPR)
,
2
Safety of Product Department
,
Grupo Boticário
,
3
Department of Genetics
,
Federal University of Paraná (UFPR)
Chapters
00:04
Introduction
01:02
Plating the Cells in 96-Well Plates
02:01
Exposing the Cells to Test Chemicals
03:19
Performing the Cytotoxicity Assays
05:51
Results: Calculation of Cell Viability for Different Test Chemicals
07:39
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议介绍了常用的细胞毒性测定(阿拉马尔蓝 [AB]、CFDA-AM、中性红和 MTT 测定),适用于评估斑马鱼胚胎 (ZEM2S) 和肝脏 (ZFL) 细胞系的细胞毒性 96 孔板。
Tags
Cytotoxicity Assay
Zebrafish Cell Lines
ZEM2S
ZFL
96-well Plate
Cell Viability
Acute Toxicity
Ecotoxicity
Alternative Fish Assays
Hepatocytes
Embryonic Cells
Cell Suspension
Multi-channel Pipette
Test Chemicals
Triton X-100
Exposure Media
Article
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