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Cytotoxicity Assays with Zebrafish Cell Lines
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Cytotoxicity Assays with Zebrafish Cell Lines

Cytotoxicity Assays with Zebrafish Cell Lines

English

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5,268 Views

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08:22 min

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January 06, 2023

DOI:

10.3791/64860-v

DOI:
10.3791/64860-v

•

08:22 min
January 06, 2023

•

41 Views
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1Graduate Program in Genetics, Department of Genetics,Federal University of Paraná (UFPR), 2Safety of Product Department,Grupo Boticário, 3Department of Genetics,Federal University of Paraná (UFPR)

Summary

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This protocol presents commonly used cytotoxicity assays (Alamar Blue [AB], CFDA-AM, Neutral Red, and MTT assays) adapted for the assessment of cytotoxicity in zebrafish embryo (ZEM2S) and liver (ZFL) cell lines in 96-well plates.

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Rodrigues de Souza, I., Wilke Sivek, T., Vaz de Oliveira, J. B., Di Pietro Micali Canavez, A., de Albuquerque Vita, N., Cigaran Schuck, D., Rodrigues de Souza, I., Cestari, M. M., Lorencini, M., Leme, D. M. Cytotoxicity Assays with Zebrafish Cell Lines. J. Vis. Exp. (191), e64860, doi:10.3791/64860 (2023).

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