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Ensayos de citotoxicidad con líneas celulares de pez cebra
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JoVE Journal Environment
Cytotoxicity Assays with Zebrafish Cell Lines
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Ensayos de citotoxicidad con líneas celulares de pez cebra

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5,275 Views

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08:22 min

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January 06, 2023

DOI:

10.3791/64860-v

DOI:
10.3791/64860-v

•

08:22 min
January 06, 2023

•

41 Views
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1Graduate Program in Genetics, Department of Genetics,Federal University of Paraná (UFPR), 2Safety of Product Department,Grupo Boticário, 3Department of Genetics,Federal University of Paraná (UFPR)

Summary

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Este protocolo presenta ensayos de citotoxicidad de uso común (ensayos Alamar Blue [AB], CFDA-AM, Neutral Red y MTT) adaptados para la evaluación de la citotoxicidad en líneas celulares de embrión de pez cebra (ZEM2S) e hígado (ZFL) en placas de 96 pocillos.

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Rodrigues de Souza, I., Wilke Sivek, T., Vaz de Oliveira, J. B., Di Pietro Micali Canavez, A., de Albuquerque Vita, N., Cigaran Schuck, D., Rodrigues de Souza, I., Cestari, M. M., Lorencini, M., Leme, D. M. Cytotoxicity Assays with Zebrafish Cell Lines. J. Vis. Exp. (191), e64860, doi:10.3791/64860 (2023).

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