Journal
/
Medio ambiente
/
Cytotoxicity Assays with Zebrafish Cell Lines
JoVE Revista
Medio ambiente
Author Produced
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Medio ambiente
Cytotoxicity Assays with Zebrafish Cell Lines

Cytotoxicity Assays with Zebrafish Cell Lines

5,268 Views

•

08:22 min

•

January 06, 2023

DOI:

10.3791/64860-v

DOI:
10.3791/64860-v

•

08:22 min
January 06, 2023

•

41 Views
, , , , , , , , ,
1Graduate Program in Genetics, Department of Genetics,Federal University of Paraná (UFPR), 2Safety of Product Department,Grupo Boticário, 3Department of Genetics,Federal University of Paraná (UFPR)

Summary

Automatically generated

This protocol presents commonly used cytotoxicity assays (Alamar Blue [AB], CFDA-AM, Neutral Red, and MTT assays) adapted for the assessment of cytotoxicity in zebrafish embryo (ZEM2S) and liver (ZFL) cell lines in 96-well plates.

Explore More Videos

Cytotoxicity AssayZebrafish Cell LinesZEM2SZFL96-well PlateCell ViabilityAcute ToxicityEcotoxicityAlternative Fish AssaysHepatocytesEmbryonic CellsCell SuspensionMulti-channel PipetteTest ChemicalsTriton X-100Exposure Media

Read Article

Cite this Article

Rodrigues de Souza, I., Wilke Sivek, T., Vaz de Oliveira, J. B., Di Pietro Micali Canavez, A., de Albuquerque Vita, N., Cigaran Schuck, D., Rodrigues de Souza, I., Cestari, M. M., Lorencini, M., Leme, D. M. Cytotoxicity Assays with Zebrafish Cell Lines. J. Vis. Exp. (191), e64860, doi:10.3791/64860 (2023).

Download .ris file

Copy

Share Video

.

Copy