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Cytotoxicity Assays with Zebrafish Cell Lines
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January 06, 2023
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This protocol presents commonly used cytotoxicity assays (Alamar Blue [AB], CFDA-AM, Neutral Red, and MTT assays) adapted for the assessment of cytotoxicity in zebrafish embryo (ZEM2S) and liver (ZFL) cell lines in 96-well plates.
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Rodrigues de Souza, I., Wilke Sivek, T., Vaz de Oliveira, J. B., Di Pietro Micali Canavez, A., de Albuquerque Vita, N., Cigaran Schuck, D., Rodrigues de Souza, I., Cestari, M. M., Lorencini, M., Leme, D. M. Cytotoxicity Assays with Zebrafish Cell Lines. J. Vis. Exp. (191), e64860, doi:10.3791/64860 (2023).
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