Journal
/
Environment
/
A Rapid and Efficient Method for Assessing Pathogenicity of Ustilago maydis on Maize and Teosinte Lines
JoVE Journal
Environment
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Environment
A Rapid and Efficient Method for Assessing Pathogenicity of Ustilago maydis on Maize and Teosinte Lines

A Rapid and Efficient Method for Assessing Pathogenicity of Ustilago maydis on Maize and Teosinte Lines

DOI:
10.3791/50712-v

•

07:09 min

•

January 03, 2014

•

,
1Department of Plant Pathology,University of Georgia

Chapters

  • 00:05Title
  • 01:13Growth of Plant Material
  • 02:33Needle Injection Inoculation and Resistance Reaction Screening
  • 05:51Representative Pathogen Resistance Results
  • 06:46Conclusion

Summary

Automatic Translation

Automatic Translation

The use of a needle injection method to inoculate maize and teosinte plants with the biotrophic pathogen Ustilago maydis is described. The needle injection inoculation method facilitates the controlled delivery of the fungal pathogen in between the plant leaves where the pathogen enters the plant through the formation of appresoria. This method is highly efficient, enabling reproducible inoculations with U. maydis.

Tags

Keywords: Ustilago MaydisMaizeTeosinteIntrogression LinesNeedle Injection InoculationBiotrophic PathogenCorn SmutResistance Screening

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/50712?article_type=v

Use of Chironomidae (Diptera) Surface-Floating Pupal Exuviae as a Rapid Bioassessment Protocol for Water Bodies
check_url/50712?article_type=v

The Use of an Automated System (GreenFeed) to Monitor Enteric Methane and Carbon Dioxide Emissions from Ruminant Animals
check_url/50712?article_type=v

Administering and Detecting Protein Marks on Arthropods for Dispersal Research
check_url/50712?article_type=v

Reliable Method for Assessing Seed Germination, Dormancy, and Mortality under Field Conditions
check_url/50712?article_type=v

Protocol for Assessing the Relative Effects of Environment and Genetics on Antler and Body Growth for a Long-lived Cervid
check_url/50712?article_type=v

Preparation of DMMTAV and DMDTAV Using DMAV for Environmental Applications: Synthesis, Purification, and Confirmation
check_url/50712?article_type=v

The Hawaii Protocol for Scientific Monitoring of Coffee Berry Borer: a Model for Coffee Agroecosystems Worldwide
check_url/50712?article_type=v

A Flexible Low Cost Hydroponic System for Assessing Plant Responses to Small Molecules in Sterile Conditions
check_url/50712?article_type=v

High-throughput, Microscale Protocol for the Analysis of Processing Parameters and Nutritional Qualities in Maize (Zea mays L.)
check_url/50712?article_type=v

Assessing the Particulate Matter Removal Abilities of Tree Leaves

Read Article