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Saggi di citotossicità con linee cellulari di zebrafish
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Cytotoxicity Assays with Zebrafish Cell Lines
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Saggi di citotossicità con linee cellulari di zebrafish

italiano

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5,275 Views

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08:22 min

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January 06, 2023

DOI:

10.3791/64860-v

DOI:
10.3791/64860-v

•

08:22 min
January 06, 2023

•

41 Views
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1Graduate Program in Genetics, Department of Genetics,Federal University of Paraná (UFPR), 2Safety of Product Department,Grupo Boticário, 3Department of Genetics,Federal University of Paraná (UFPR)

Summary

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Questo protocollo presenta saggi di citotossicità comunemente usati (Alamar Blue [AB], CFDA-AM, Neutral Red, e MTT assays) adattati per la valutazione della citotossicità in linee cellulari di embrioni di zebrafish (ZEM2S) e fegato (ZFL) in piastre a 96 pozzetti.

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Rodrigues de Souza, I., Wilke Sivek, T., Vaz de Oliveira, J. B., Di Pietro Micali Canavez, A., de Albuquerque Vita, N., Cigaran Schuck, D., Rodrigues de Souza, I., Cestari, M. M., Lorencini, M., Leme, D. M. Cytotoxicity Assays with Zebrafish Cell Lines. J. Vis. Exp. (191), e64860, doi:10.3791/64860 (2023).

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