Journal
/
Environment
/
ゼブラフィッシュ細胞株を用いた細胞毒性アッセイ
JoVE Journal
Environment
Author Produced
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Environment
Cytotoxicity Assays with Zebrafish Cell Lines
Please note that all translations are automatically generated. Click here for the English version.

ゼブラフィッシュ細胞株を用いた細胞毒性アッセイ

日本語

Automatically Generated

5,275 Views

•

08:22 min

•

January 06, 2023

DOI:

10.3791/64860-v

DOI:
10.3791/64860-v

•

08:22 min
January 06, 2023

•

41 Views
, , , , , , , , ,
1Graduate Program in Genetics, Department of Genetics,Federal University of Paraná (UFPR), 2Safety of Product Department,Grupo Boticário, 3Department of Genetics,Federal University of Paraná (UFPR)

Summary

Automatically generated

このプロトコルは、96ウェルプレートのゼブラフィッシュ胚(ZEM2S)および肝臓(ZFL)細胞株の細胞毒性の評価に適した、一般的に使用される細胞毒性アッセイ(Alamar Blue [AB]、CFDA-AM、ニュートラルレッド、およびMTTアッセイ)を提示します。

Explore More Videos

Cytotoxicity AssayZebrafish Cell LinesZEM2SZFL96-well PlateCell ViabilityAcute ToxicityEcotoxicityAlternative Fish AssaysHepatocytesEmbryonic CellsCell SuspensionMulti-channel PipetteTest ChemicalsTriton X-100Exposure Media

Read Article

Cite this Article

Rodrigues de Souza, I., Wilke Sivek, T., Vaz de Oliveira, J. B., Di Pietro Micali Canavez, A., de Albuquerque Vita, N., Cigaran Schuck, D., Rodrigues de Souza, I., Cestari, M. M., Lorencini, M., Leme, D. M. Cytotoxicity Assays with Zebrafish Cell Lines. J. Vis. Exp. (191), e64860, doi:10.3791/64860 (2023).

Download .ris file

Copy

Share Video

.

Copy