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Journal
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Immunology and Infection
/
使用肽-泊洛沙明纳米颗粒在培养细胞中高效转染
体外
转录的mRNA
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Efficient Transfection of
In vitro
Transcribed mRNA in Cultured Cells Using Peptide-Poloxamine Nanoparticles
Please note that all translations are automatically generated.
Click here for the English version.
使用肽-泊洛沙明纳米颗粒在培养细胞中高效转染
体外
转录的mRNA
DOI:
10.3791/64288-v
•
10:16 min
•
August 17, 2022
•
Qin Xiao*
1
,
Yuheng Liu*
1
,
Dandan Zhang
,
Chao Li
,
Qihua Yang
,
Dongshui Lu
,
Weijun Zhang
,
Joseph Rosenecker
,
Quanming Zou
,
Yang Li
,
Shan Guan
1
National Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy
,
Third Military Medical University
,
2
Department of Pediatrics
,
Ludwig-Maximilians University of Munich
,
3
Department of Pharmacy, Southwest Hospital
,
Third Military Medical University
Chapters
00:05
Introduction
01:19
In Vitro Transcription (IVT) of Chemically Modified mRNA
02:55
Generation of IVT mRNA/PP-sNps
05:22
Preparation of the Cells for Transfection and Transfection of the Cultured Cells
07:04
Analysis of Cell Transfection Efficacy Using Metridia Luciferase (MetLuc) Assay
07:53
Results: Analyzing the Transfection Efficiency of MetLuc-mRNA/PP-sNp
09:12
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
使用微流体混合装置开发自组装肽 - 泊洛沙明纳米颗粒(PP-sNp)以封装和递送
体外
转录的信使RNA。所描述的mRNA / PP-sNp可以在
体外
有效地转染培养的细胞。
Tags
MRNA Transfection
Peptide-poloxamine Nanoparticles
Mucosal MRNA Vaccine
Lung Cancer
Cystic Fibrosis
In Vitro Transcription
Capped MRNA
Agarose Gel Electrophoresis
Poloxamine 704
Synthetic Peptide
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