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Genotypic Inference of HIV-1 Tropism Using Population-based Sequencing of V3
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Genotypic Inference of HIV-1 Tropism Using Population-based Sequencing of V3

Genotypic Inference of HIV-1 Tropism Using Population-based Sequencing of V3

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12,002 Views

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11:10 min

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December 27, 2010

DOI:

10.3791/2531-v

DOI:
10.3791/2531-v

•

11:10 min
December 27, 2010

•

3 Views
, ,
1Laboratory Program,BC Centre for Excellence in HIV/AIDS

Summary

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HIV tropism can be inferred from the V3 region of the viral envelope. V3 is PCR amplified in triplicate using nested RT-PCR, sequenced, and interpreted using bioinformatic software. Samples with with 1 or more sequence(s) with low g2P scores are classified as non-R5 virus.

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McGovern, R. A., Harrigan, P. R., Swenson, L. C. Genotypic Inference of HIV-1 Tropism Using Population-based Sequencing of V3. J. Vis. Exp. (46), e2531, doi:10.3791/2531 (2010).

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