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Immunology and Infection
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Quantitative In vitro Assay to Measure Neutrophil Adhesion to Activated Primary Human Microvascular Endothelial Cells under Static Conditions
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Immunology and Infection
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JoVE Journal Immunology and Infection
Quantitative In vitro Assay to Measure Neutrophil Adhesion to Activated Primary Human Microvascular Endothelial Cells under Static Conditions

Quantitative In vitro Assay to Measure Neutrophil Adhesion to Activated Primary Human Microvascular Endothelial Cells under Static Conditions

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16,948 Views

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11:22 min

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August 23, 2013

DOI:

10.3791/50677-v

DOI:
10.3791/50677-v

•

11:22 min
August 23, 2013

•

4 Views
, ,
1Department of Anesthesia and Perioperative Care,University of California, San Francisco, 2Graduate Program in Biomedical Sciences,University of California, San Francisco

Summary

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Neutrophil adherence to the activated endothelium at sites of infection is an integral component of the host's inflammatory response. Described in this report is a neutrophil binding assay that allows for the in vitro quantitation of primary human neutrophil binding to endothelial cells activated by inflammatory mediators under static conditions.

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Wilhelmsen, K., Farrar, K., Hellman, J. Quantitative In vitro Assay to Measure Neutrophil Adhesion to Activated Primary Human Microvascular Endothelial Cells under Static Conditions. J. Vis. Exp. (78), e50677, doi:10.3791/50677 (2013).

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