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Journal
/
Immunology and Infection
/
Retroviral Transduction of T-cell Receptors in Mouse T-cells
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Retroviral Transduction of T-cell Receptors in Mouse T-cells
Retroviral Transduction of T-cell Receptors in Mouse T-cells
DOI:
10.3791/2307-v
•
10:14 min
•
October 22, 2010
•
Shi Zhong
,
Karolina Malecek
2
,
Arianne Perez-Garcia
,
Michelle Krogsgaard
1
NYU Cancer institute
,
New York University School of Medicine
,
2
Program in Structural Biology
,
New York University School of Medicine
Chapters
00:00
Title
01:37
Retroviral Transfection and T-cell Isolation
03:55
Prepapration of a Single-cell Suspension from Mouse Spleen
04:51
Isolation and Activation of Mouse T-cells
06:27
Infection of Activated T-cells
08:21
Expansion of Cells and Evaluation of Transduction Efficiency
08:55
Retroviral Expression of TCR on Mouse T-cells
09:32
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
We present a protocol to produce antigen-specific mouse T-cells using retroviral transduction
Tags
Retroviral Transduction
T-cell Receptors
Mouse T-cells
Immune System
Peptide Antigens
Antigen Presenting Cells
Functional Outcomes
Immunology Related Diseases
TCR Transgenic Mouse Models
Bone Marrow Chimera
Retroviral Transduction Method
Peptide-MHC Specificity
Downstream Applications
Human Immunotherapy
Adoptive Transfer
Cancer Treatment
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