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Efficient Transfection of In vitro Transcribed mRNA in Cultured Cells Using Peptide-Poloxamine Nanoparticles
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Efficient Transfection of In vitro Transcribed mRNA in Cultured Cells Using Peptide-Poloxamine Nanoparticles
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August 17, 2022
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Summary
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A self-assembled peptide-poloxamine nanoparticle (PP-sNp) is developed using a microfluidic mixing device to encapsulate and deliver in vitro transcribed messenger RNA. The described mRNA/PP-sNp could efficiently transfect cultured cells in vitro.
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Xiao, Q., Liu, Y., Zhang, D., Li, C., Yang, Q., Lu, D., Zhang, W., Rosenecker, J., Zou, Q., Li, Y., Guan, S. Efficient Transfection of In vitro Transcribed mRNA in Cultured Cells Using Peptide-Poloxamine Nanoparticles. J. Vis. Exp. (186), e64288, doi:10.3791/64288 (2022).
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