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A Simple and Efficient Approach to Construct Mutant Vaccinia Virus Vectors
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October 30, 2016
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1Center for Molecular Oncology, Barts Cancer Institute,Queen Mary University of London, 2Sino-British Research Centre for Molecular Oncology, National Center for International Research in Cell and Gene Therapy,Zhengzhou University, 3School of Basic Medical Sciences, Academy of Medical Sciences,Zhengzhou University
Summary
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Vaccinia virus (VV) has been widely used in biomedical research and the improvement of human health. This article describes a simple, highly efficient method to edit the VV genome using a CRISPR-Cas9 system.
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Yuan, M., Wang, P., Chard, L. S., Lemoine, N. R., Wang, Y. A Simple and Efficient Approach to Construct Mutant Vaccinia Virus Vectors. J. Vis. Exp. (116), e54171, doi:10.3791/54171 (2016).
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