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Trasfezione efficiente di mRNA trascritto in vitro in cellule in coltura utilizzando nanoparticelle peptide-poloxamina
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Immunology and Infection
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JoVE Journal Immunology and Infection
Efficient Transfection of In vitro Transcribed mRNA in Cultured Cells Using Peptide-Poloxamine Nanoparticles
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Trasfezione efficiente di mRNA trascritto in vitro in cellule in coltura utilizzando nanoparticelle peptide-poloxamina

italiano

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2,917 Views

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10:16 min

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August 17, 2022

DOI:

10.3791/64288-v

DOI:
10.3791/64288-v

•

10:16 min
August 17, 2022

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8 Views
, , , , , , , , , ,
1National Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy,Third Military Medical University, 2Department of Pediatrics,Ludwig-Maximilians University of Munich, 3Department of Pharmacy, Southwest Hospital,Third Military Medical University

Summary

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Una nanoparticella peptide-poloxamina autoassemblata (PP-sNp) viene sviluppata utilizzando un dispositivo di miscelazione microfluidica per incapsulare e fornire RNA messaggero trascritto in vitro . L'mRNA/PP-sNp descritto potrebbe trasfettare efficacemente le cellule in coltura in vitro.

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Cite this Article

Xiao, Q., Liu, Y., Zhang, D., Li, C., Yang, Q., Lu, D., Zhang, W., Rosenecker, J., Zou, Q., Li, Y., Guan, S. Efficient Transfection of In vitro Transcribed mRNA in Cultured Cells Using Peptide-Poloxamine Nanoparticles. J. Vis. Exp. (186), e64288, doi:10.3791/64288 (2022).

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