Journal
/
Biochemistry
/
Fingerprinting Cardiolipin in Leukocytes by Mass Spectrometry for a Rapid Diagnosis of Barth Syndrome
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
Fingerprinting Cardiolipin in Leukocytes by Mass Spectrometry for a Rapid Diagnosis of Barth Syndrome

Fingerprinting Cardiolipin in Leukocytes by Mass Spectrometry for a Rapid Diagnosis of Barth Syndrome

English

Automatically Generated

2,196 Views

•

06:48 min

•

March 23, 2022

DOI:

10.3791/63552-v

DOI:
10.3791/63552-v

•

06:48 min
March 23, 2022

•

2 Views
, , ,
1Medical School,Swansea University, 2Department of Basic Medical Sciences, Neuroscience and Sense Organs,University of Bari Aldo Moro

Summary

Automatically generated

This protocol shows how to obtain a mass spectrometric "fingerprint" of leukocyte cardiolipin for the diagnosis of Barth syndrome. The assessment of elevated monolysocardiolipin to cardiolipin ratio discriminates patients with Barth syndrome from control heart failure patients with 100% sensitivity and specificity.

Explore More Videos

Cardiolipin FingerprintingLeukocytesMass SpectrometryBarth SyndromeMonolysocardiolipinMALDI-TOFBlood Sample ProcessingOsmotic ShockLipid Extraction

Read Article

Cite this Article

Angelini, R., Russo, S., Corcelli, A., Lobasso, S. Fingerprinting Cardiolipin in Leukocytes by Mass Spectrometry for a Rapid Diagnosis of Barth Syndrome. J. Vis. Exp. (181), e63552, doi:10.3791/63552 (2022).

Download .ris file

Copy

Share Video

.

Copy